Morphological and glycan features of the camel oviduct epithelium

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• 作者：Gianluca Accogli^a ; Davide Monaco^a ; Khalid Ahmed El Bahrawy^b ; Ashraf Abd El-Halim El-Sayed^c ; ^d ; Francesca Ciannarella^a ; Benedicte Beneult^e ; Giovanni Michele Lacalandra^a ; Salvatore Desantis^a ; ^{salvatore.desantis@uniba.it" class="auth_mail}
• 关键词：Oviduct ; Glycoproteins ; Sialic acid ; Lectin histochemistry ; SEM ; Camel ; Follicular wave
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：July 2014
• 年：2014
• 卷：196
• 期：4
• 页码：197-205
• 全文大小：3206 K

文摘

This study describes regional differences in the oviduct of the one-humped camel (Camelus dromedarius) during the growth phase (GP) and the mature phase (MP) of the follicular wave by means of morphometry, scanning electron microscopy (SEM) and glycohistochemistry investigations. Epithelium height significantly increased in the ampulla and decreased in the isthmus passing from the GP to the MP. Under SEM, non-ciliated cells displayed apical blebs (secretory) or short microvilli. Cilia glycocalyx expressed glycans terminating with sialic acid linked 伪2,6 to Gal/GalNAc (SNA affinity) throughout the oviducts of GP and MP and sialic acid linked 伪2,3 to Gal尾1,3GalNAc (MAL II and KOH-sialidase (K-s)-PNA staining) throughout the MP oviducts. Non-ciliated cells displayed lectin-binding sites from the supra-nuclear cytoplasm to the luminal surface. Ampulla non-ciliated cells showed O-linked (mucin-type) sialoglycans (MAL II and K-s-PNA) during GP and MP and N-linked sialoglycans (SNA) during the MP. Isthmus non-ciliated cells expressed SNA reactivity in GP and MP, also K-s-PNA binders in MP, and MAL II and PNA affinity (Gal尾1,3GalNAc) during GP. Gal尾1,3GalNAc was sialilated in the non-ciliated cells of GP UTJ. Luminal surface lacked of Gal尾1,3GalNAc in GP and MP, whereas it expressed 伪2,6- and 伪2,3-linked sialic acids. In GP intraluminal substance reacted with SNA, MAL II, K-s-PNA in ampulla and only with MAL II in the isthmus and UTJ. These results demonstrate that the morphology and the glycan pattern of the camel oviductal epithelium vary during the follicular wave and that could relate to the region-specific functions.