Association of CK19 mRNA detection of occult cancer cells in mediastinal lymph nodes in non-small cell lung carcinoma and high risk of early recurrence

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• 作者：Pimpec-Barthes ; Fran??oise Le ; Danel ; Claire ; Lacave ; Roger ; Ricci ; Sylvie ; Bry ; Xavier ; et. al.
• 关键词：Cytokeratin ; Reverse transcriptase polymerase chain reaction ; Metastasis ; Lung neoplasm ; Thoracic surgery ; Lymph node dissection ; Mediastinum ; Prognosis
• 刊名：European Journal of Cancer
• 出版年：2005
• 出版时间：January, 2005
• 年：2005
• 卷：41
• 期：2
• 页码：306-312
• 全文大小：291 K

文摘

This study was designed to screen occult cancer cells by CK19 mRNA detection using reverse transcriptase-polymerase chain reaction (RT-PCR) in mediastinal lymph nodes stations (MLNS) in non-small cell lung carcinoma (NSCLC). In 49 NSCLC patients free of mediastinal adenopathy on computed tomograph, 254 MLNS were evaluated by histopathology, immunohistochemistry (IHC) and RT-PCR. Of 225 non-tumoral MLNS on histopathology, 32 (14.2 % ) were positive by RT-PCR. IHC did not provide significant additional results. Seventeen patients were without mediastinal tumoral extension on histopathology and RT-PCR (Group 1), 16 were upgraded by RT-PCR (Group 2) and 16 pN2 on histopathology (Group 3). The two-year cancer-related death survival in Groups 1 (100 % ) and 2 (64.5 % ) was significantly different (P