Determining the Turnover of Glycosphingolipid Species by Stable-Isotope Tracer Lipidomics

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• 作者：Tore Skotland¹ ; ² ; ^&dagger ; ; ^{Tore.Skotland@rr-research.no} ; Kim Ekroos³ ; ^&dagger ; ; ^{kekroos@hotmail.com} ; Simona Kavaliauskiene¹ ; ² ; ⁴ ; Jonas Bergan¹ ; ² ; Dimple Kauhanen³ ; Tuulia Lintonen³ ; Kirsten Sandvig¹ ; ² ; ⁴
• 关键词：MS ; mass spectrometry ; GSL ; glycosphingolipid ; Cer ; ceramide ; Gb3 ; globotriaosylceramide ; GlcCer ; glucosylceramide ; LacCer ; lactosylceramide ; MRM ; multiple reaction monitoring
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：428
• 期：24
• 页码：4856-4866
• 全文大小：1068 K
• 卷排序：428

文摘

Mass isotope labeling combined with lipidomics determines turnover of molecular GLSs. Rapid and differential turnover of lipid classes and their individual species were observed. Long-chain GSL species showed a more rapid turnover than the very-long-chain species. The analyses revealed different cellular pools of glucosylceramide, lactosylceramide, and globotriaosylceramide. Mass spectrometry analyses of cells labeled with [13C]glucose are a powerful tool to study GSL turnover.