Detection of egg and milk residues on working surfaces by ELISA and lateral flow immunoassay tests

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• 作者：Patricia Galan-Malo^a ; Marí ; a Ló ; pez^b ; Juan-Carlos Ortiz^c ; Marí ; a D. Pé ; rez^b ; Lourdes Sá ; nchez^b ; Pedro Razquin^a ; Luis Mata^a ; ^{lmata@zeulab.com}
• 关键词：Cross contact ; Surfaces control ; Swab ; Hidden allergens ; Beta-lactoglobulin ; Ovalbumin
• 刊名：Food Control
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：45-53
• 全文大小：1798 K
• 卷排序：74

文摘

Optimization of a procedure for allergen control on surfaces by combination of a swabbing method and two immunochemical tests. Selection of swab material and extraction buffers influence the recovery of egg and milk residues. Swabbing method should be adapted to the kind of surface and the detection method. Quantitative ELISA method can be used for the validation of cleaning procedures in food industry facilities. Qualitative lateral flow test is an easy and suitable method for routine verification of cleaning effectiveness.