Developmental expression of B cell molecules in equine lymphoid tissues

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• 作者：J.M.B. Prieto ^{jmb279@cornell.edu} ; R.L. Tallmadge ^{rlt8@cornell.edu} ; M.J.B. Felippe ; ^{mbf6@cornell.edu}
• 关键词：CDR ; complementary determining region ; DG ; days of gestation ; DGKA ; diacylglycerol kinase alpha ; FCS ; fetal calf serum ; FGL2 ; fibrinogen-like protein 2 ; Ig ; immunoglobulin ; IGK ; Ig kappa light chain ; IGL ; Ig lambda light chain ; IGLJ ; Ig lambda joining gene ; IGLV ; Ig lambda variable gene ; IGHM ; Ig mu heavy chain ; IL-10 ; interleukin-10 ; MHC ; major histocompatibility complex ; MFI ; mean fluorescence intensity ; N-nucleotide ; non-template nucleotide ; PAX5 ; paired box 5 ; PBS ; phosphate buffered solutio
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：183
• 期：Complete
• 页码：60-71
• 全文大小：1605 K
• 卷排序：183

文摘

Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5hi B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5hi and CD5lo B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5hi B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.