Development of recombinant antibody-based enzyme-linked immunosorbent assay (ELISA) for the detection of skatole

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• 作者：Janne Leivo ; ^{jpleiv@utu.fi" class="auth_mail" title="E-mail the corresponding author} ; Joonas Mä ; kelä ; Jaana Rosenberg ; Urpo Lamminmä ; ki
• 关键词：ELISA ; Boar taint ; Immunoassay ; Phage display ; Antibody engineering ; Hapten
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：492
• 期：Complete
• 页码：27-29
• 全文大小：320 K

文摘

The occurrence of boar taint and the European Commission recommendation to discontinue the surgical castration of pigs by the year 2018 creates an urgent need for new analytical methods that are simple, affordable, and suitable for field testing. We describe the generation and engineering of a skatole-specific antibody derived from a synthetic antibody library and the development of ELISA for its detection. The immunoassay is capable of detecting skatole with IC50 of 222 μg L⁻¹, which is within the analytical threshold level suggested for skatole, and with low cross-reactivity interference from other indolic compounds.