Estrogenic and dioxin-like compounds in sediment from Zierikzee harbour identified with CALUX assay-directed fractionation combined with one and two dimensional gas chromatography analyses

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• 作者：Corine J. Houtman ; Petra Booij ; Eric Jover ; David Pascual del Rio ; Kees Swart ; Martin van Velzen ; Rene Vreuls ; Juliette Legler ; Abraham Brouwer ; Marja H. Lamoree
• 关键词：Toxicity identification and evaluation ; Bioassay-directed fractionation ; Hormones ; Polycylic aromatic hydrocarbons ; Sediment
• 刊名：Chemosphere
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：65
• 期：11
• 页码：2244-2252
• 全文大小：359 K

文摘

The identity of compounds responsible for estrogenic and dioxin-like activities in sediment from the harbour of the small town Zierikzee in Zeeland, The Netherlands, was investigated using a bioassay directed fractionation approach with the in vitro estrogen and dioxin responsive reporter gene assays ER- and DR-CALUX. For identification of compounds exhibiting activity in the bioassays, either one or two-dimensional GC in combination with quadrupole (MSD), ion trap (ITD) or time-of-flight mass spectrometric detection (ToF-MS) was used, depending on the biological and chemical characteristics and the complexity of the fractions. The natural estrogenic hormone 17-β-estradiol and its metabolite estrone were identified with GC–ITD as the main contributors to the estrogenic activity. After successive rounds of fractionation, the dioxin-like activity could be explained by the presence of various polycyclic aromatic hydrocarbons identified with GC–MSD and two-dimensional comprehensive GC × GC–ToF-MS. Some estrogenic activity of a relatively non-polar nature remained unidentified.