Effect of chitosan molecular weight on the formation of chitosan-pullulanase soluble complexes and their application in the immobilization of pullulanase onto Fe3O4-κ-carrageenan nanoparticles
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- 作者：Jie Longa ; b ; Enbo Xua ; b ; Xingfei Lia ; b ; Zhengzong Wua ; b ; Fang Wanga ; b ; Xueming Xua ; b ; Zhengyu Jina ; b ; jinlab2008@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Aiquan Jiaoa ; b ; jiaoaq@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaobei Zhanc
- 关键词：Chitosan ; Molecular weight ; Pullulanase ; Complex ; Fe3O4&ndash ; κ-carrageenan nanoparticles
- 刊名：Food Chemistry
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：202
- 期：Complete
- 页码：49-58
- 全文大小：590 K
文摘
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Complexation of chitosan and pullulanase was affected by chitosan molecular weight.
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Pullulanase was immobilized onto Fe3O4–κ-carrageenan nanoparticles by complexation.
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Interrelationship exists between chitosan Mw and binding characteristic.
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The high binding affinity induces more alterations of protein secondary structure.
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Changes in secondary structures impact enzymatic properties of immobilized enzyme.