A bifunctional xylanase (Xyn10E) from P. curdlanolyticus B-6 was newly isolated.
rXyn10E degraded xylan, β-glucan, and microcrystalline cellulose.
CBM22 of Xyn10E has important roles in the binding and degradation of β-glucan.
Structural features of CBM22 of Xyn10E are closest to CBM22-1 modules.
rXyn10E and rXyn11A showed synergistic activity toward lignocellulosic biomass.