Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus

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• 作者：Ali Bougatef ; Mohamed Hajji ; Rafik Balti ; Imen Lassoued ; Yosra Triki-Ellouz ; Moncef Nasri
• 关键词：Protein hydrolysates ; Gastrointestinal proteases ; Antioxidant activity ; Free radical-scavenging activity ; Smooth hound (Mustelus mustelus)
• 刊名：Food Chemistry
• 出版年：2009
• 出版时间：15 June 2009
• 年：2009
• 卷：114
• 期：4
• 页码：1198-1205
• 全文大小：434 K

文摘

We have investigated the antioxidative activity of five hydrolysates from smooth hound (Mustelus mustelus) meat obtained by various gastrointestinal proteases: crude enzyme extract, low molecular weight (LMW) alkaline protease and trypsin-like protease from M. mustelus intestine, pepsin from M. mustelus stomach, and bovine trypsin.

The antioxidant activities of the different smooth hound protein hydrolysates (SHPHs) were evaluated using various in vitro antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, total antioxidant capacity, lipid peroxidation inhibition in rat liver homogenate and β-carotene bleaching assay. The five hydrolysates showed different degrees of hydrolysis and varying degrees of antioxidant activity. The hydrolysate produced by the LMW protease generally showed a greater antioxidative activity as indicated by all the methods considered. The IC₅₀ values (the concentration of antioxidant substance removing 50 % of DPPH radical) for DPPH and lipid peroxidation were found to be 0.6 ± 0.01 and 1.1 ± 0.06 mg/ml, respectively. Moreover, LMW protease hydrolysate exhibited notable reducing power and strong total antioxidant capacity.

The protein hydrolysate produced by the LMW protease was then fractionated by size exclusion chromatography on a Sephadex G-25 into three major fractions (F₁–F₃). Fraction F₃, with molecular weight lower than 3500 Da, was found to display a high antioxidant activity than F₁ (12,200 Da) and F₂ (molecular weights between 6500 and 12,200 Da).

The amino acid analysis by GC/MS showed that F₃ was rich in histidine, methionine, tyrosine, leucine, Isoleucine, glycine, and arginine.