The initiation of lateral roots in the primary roots of maize (Zea mays L.) implies a reactivation of cell proliferation in a group of founder pericycle cells

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• 作者：M.Victoria Alarcó ; n^a ; Pedro. G. Lloret^b ; Gervasio Martí ; n-Partido^b ; Julio Salguero^c ; ^{salguero@unex.es" class="auth_mail" title="E-mail the corresponding author} ; ^{salguero.unex@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：BrdU ; 5-bromo-2&prime ; -deoxyuridine ; LR ; lateral root ; PFC ; pericycle founder cells ; RCJ ; root cap junction
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：105-110
• 全文大小：1027 K

文摘

The initiation of lateral roots (LRs) has generally been viewed as a reactivation of proliferative activity in pericycle cells that are committed to initiate primordia. However, it is also possible that pericycle founder cells that initiate LRs never cease proliferative activity but rather are displaced to the most distal root zones while undertaking successive stages of LR initiation. In this study, we tested these two alternative hypotheses by examining the incorporation of 5-bromo-2′-deoxyuridine (BrdU) into the DNA of meristematic root cells of Zea mays. According to the values for the length of the cell cycle and values for cell displacement along the maize root, our results strongly suggest that pericycle cells that initiate LR primordia ceased proliferative activity upon exiting the meristematic zone. This finding is supported by the existence of a root zone between 4 and 20 mm from the root cap junction, in which neither mitotic cells nor labelled nuclei were observed in phloem pericycle cells.