A calorimetric and spectroscopic comparison of the effects of cholesterol and its immediate biosynthetic precursors 7-dehydrocholesterol and desmosterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes
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- 作者：Matthew G.K. Benesch ; Ruthven N.A.H. Lewis ; Ronald N. McElhaney ; rmcelhan@ualberta.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：CHOL ; cholesterol (5-cholestene-3β-ol) ; 7DHC ; 7-dehydrocholesterol (5 ; 7-cholestadiene-3β-ol) ; DES ; desmosterol (5 ; 24-cholestadiene-3β-ol) ; LATH ; lathosterol (7-cholestene-3β-ol) ; aCHOL ; allocholesterol (4-cholestene-3β-ol) ; PC ; phosphatidylcholine ; DPPC ; dipalmitoyophosphatidylcholine ; DMPC ; dimyristoylphosphatidylcholine ; DOPC ; dioleoylphosphatidylcholine ; POPC ; 1-palmitoyl-2-oleoylphosphatidylcholine ; SpM ; sphingomyelin ; ESR ; electron spin resonance ; NMR ; nuclear magnetic resonance ; DSC ; diffe
- 刊名：Chemistry and Physics of Lipids
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：191
- 期：Complete
- 页码：123-135
- 全文大小：1751 K
文摘
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The incorporation of CHOL, 7DHC or DES produces comparable decreases in the thermal stability of gel state DPPC bilayers.
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The incorporation of CHOL and DES increase the thermal stability of fluid DPPC bilayers while the incorporation of 7DHC decreases it.
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CHOL is more laterally miscible in both gel and fluid DPPC bilayers than are 7DHC and especially DES.
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CHOL increases the order of the hydrocarbon chains of fluid DPPC bilayers to a greater extent than DES and especially 7DHC.
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The incorporation of CHOL, 7DHC or DES does not alter the degree of H-bonding of the carbonyl groups of DPPC.