Establishment of an ex vivo laticifer cell suspension culture from Taraxacum brevicorniculatum as a production system for cis-isoprene
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- 作者：Janina Posta ; janinapost@uni-muenster.de" class="auth_mail ; Wolfgang Eisenreichb ; wolfgang.eisenreich@mytum.de" class="auth_mail ; Claudia Huberb ; claudia.huber@mytum.de" class="auth_mail ; Richard M. Twymanc ; richard@twymanrm.com" class="auth_mail ; Dirk Prü ; fera ; d ; dpruefer@uni-muenster.de" class="auth_mail ; Christian Schulze Gronoverd ; christian.schulze.gronover@ime.fraunhofer.de" class="auth_mail
- 关键词：CLSM ; confocal laser scanning microscopy ; CPT ; cis-prenyltransferase ; DOSY ; diffusion ordered spectroscopy ; IPP ; isopentenyl diphosphate ; MVA ; mevalonic acid ; MVL ; mevalonolactone ; HMW ; high molecular weight ; NMR ; nuclear magnetic resonance ; NR ; natural rubber ; PPO ; polyphenol oxidase ; SRPP ; small rubber particle protein
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：May 2014
- 年：2014
- 卷：103
- 期：Complete
- 页码：85-93
- 全文大小：2186 K
文摘
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We established a laticifer cell suspension culture from T. brevicorniculatum.
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The cells accumulated high levels of cis-isoprene.
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Feeding with the isoprene precursor mevalonolactone increased cis-isoprene production by 17-fold.