Direct interaction between Teashirt and Sex combs reduced proteins, via Tsh's acidic domain, is essential for specifying the identity of the prothorax in Drosophila

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• 作者：Ouarda Taghli-Lamallem ; Armel Gallet ; Fré ; deric Leroy ; Pascale Malapert ; Christine Vola ; Stephen Kerridge ; Laurent Fasano
• 关键词：Teashirt ; Sex combs reduced ; Hox ; Prothoracic identity ; Trunk ; Transcriptional repression ; Drosophila
• 刊名：Developmental Biology
• 出版年：2007
• 出版时间：1 July 2007
• 年：2007
• 卷：307
• 期：1
• 页码：142-151
• 全文大小：1996 K

文摘

teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We used the development of the first thoracic segment as a paradigm for Scr dependent regional morphological distinctions. In this specific context, we asked whether Tsh protein could have a direct influence on Scr activity. Here we present evidence that Tsh interacts directly with Scr and this interaction depends in part on the presence of a short domain located in the N-terminal half of Teashirt called “acidic domain”. In vivo, expression of full length Tsh can rescue the tsh null phenotype throughout the trunk whereas Tsh lacking the Scr interacting domain rescues all the trunk defects except in the prothorax. We suggest this provides insights into the mechanism by which Tsh, in concert with Scr, specifies the prothoracic identity.