Stepwise functionalization of SiN_x

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• 作者：Sté ; phanie Dauphas ; Soraya Ababou-Girard ; Auré ; lie Girard ; France Le Bihan ; Tayeb Mohammed-Brahim ; Vé ; ronique Vié ; Anne Corlu ; Christiane Guguen-Guillouzo ; Olivier Lavastre ; Florence
• 关键词：Silane ; Surface functionalization ; Silicon nitride ; Transferrin ; Atomic force microscopy ; X-ray photoelectron spectroscopy
• 刊名：Thin Solid Films
• 出版年：2009
• 出版时间：1 September 2009
• 年：2009
• 卷：517
• 期：21
• 页码：6016-6022
• 全文大小：1238 K

文摘

A stepwise functionalization of silicon nitride surfaces is followed by X-ray photoelectron spectroscopy (XPS). The first step involves a silanization reaction leading to the formation of a silane film with a thickness estimated by XPS of one or two molecular layers. A monoprotected homobifunctionalized linker is then used to avoid the formation of bridge structures on the surface. The linker reacts quantitatively with the amino groups of the surface as outlined by the absence of residual unreacted CNH₂/CNH₃⁺ groups in XPS analyses. Deprotection of the ester groups of the immobilized linker and subsequent reaction with N-hydroxysuccinimid lead to N-hydroxysuccinimid activated surfaces able to react with biological species. These surfaces were then incubated with anti-transferrin antibodies. As seen by XPS and atomic force microscopy analyses, the concentration and incubation conditions of antibodies are important to obtain a compact layer of antibodies on the surface. All chemical steps of the procedure are compatible with microelectronic process on silicon. Moreover, antibodies introduced under native conditions at physiological pH, in the last step of the immobilization process, recognized specifically antigens, as shown by fluorescence competitive assay.