Development of live-attenuated arenavirus vaccines based on codon deoptimization of the viral glycoprotein

详细信息    查看全文

• 作者：Benson Y.H. Cheng^a ; ¹ ; Aitor Nogales^a ; ¹ ; Juan Carlos de la Torre^b ; Luis Martí ; nez-Sobrido^a ; ^{luis_martinez@urmc.rochester.edu}
• 关键词：Arenavirus ; Lymphocytic choriomeningitis virus ; Live-attenuated vaccines ; Codon deoptimization ; Glycoprotein ; Codon usage ; Reverse genetics
• 刊名：Virology
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：501
• 期：Complete
• 页码：35-46
• 全文大小：2716 K
• 卷排序：501

文摘

Codon deoptimization (CD) results in reduced LCMV GP expression levels. A rLCMV encoding a CD GP (rLCMV/CD) shows WT-like growth properties in vitro. rLCMV/CD is highly attenuated, as compared to LCMV WT, in vivo. rLCMV/CD induces complete protection against a lethal challenge with rLCMV/WT. A GP CD-based strategy can be implement for the development of arenavirus vaccines.