Shaken flasks by resonant acoustic mixing versus orbital mixing: Mass transfer coefficient kLa characterization and Escherichia coli cultures comparison
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- 作者：Greta I. Reynoso-Cereceda gretareynoso@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Ramses I. Garcia-Cabrera ramses@biomedicas.unam.mx" class="auth_mail" title="E-mail the corresponding author ; Norma A. Valdez-Cruz adrivaldez1@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Mauricio A. Trujillo-Roldá ; n ; maurotru@gmail.com" class="auth_mail" title="E-mail the corresponding author ; maurotru@biomedicas.unam.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：A ; superficial area of the liquid at the inner diameter of the motionless flask ; cm2 ; a ; b ; c ; coefficients for the empirical models specific for each agitation system ; adj R2 ; adjusted R-square ; A/V ; superficial area/filling volume ratio ; cm&minus ; 1 ; CL* ; oxygen concentration at the interfacial saturation ; CL ; oxygen concentration in the liquid bulk ; d ; maximum inner flask diameter ; m ; d0 ; shaking diameter ; cm ; DOT ; dissolved oxygen tension ; % air saturation ; DOF ; degrees of freedom ; Fr ; Froude
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：105
- 期：part_PB
- 页码：379-390
- 全文大小：2342 K
文摘
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Resonant acoustic mixing (RAM) for shaken flasks is characterized in terms of the kLa.
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Empirical models for kLa in shaken flasks by RAM and orbital mixing are presented.
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E. coli cultures show similar kinetics in RAM and orbital mixing at kLa of 46 h−1.
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At kLa of 92 h−1E. coli exhibited differences in DOT profiles and culture parameters.