Quantitative evaluation of alternatively spliced mRNA isoforms by label-free real-time plasmonic sensing
详细信息 查看全文
- 作者：Cé ; sar S. Huertasa ; L.G. Carrascosaa ; S. Bonnalb ; c ; J. Valcá ; rcelb ; c ; d ; L.M. Lechugaa ; laura.lechuga@cin2.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Alternative splicing ; mRNA isosoforms ; SPR ; Biosensor ; Biomarker ; Diagnosis
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：78
- 期：Complete
- 页码：118-125
- 全文大小：1379 K
文摘
- •
A direct and label-free biosensor for alternative splicing monitoring is reported.
- •
Isoform discrimination reduced cross-hybridization to background levels.
- •
RNA hydrolysis aided target accessibility and establishment of a general methodology.
- •
Versatility of the methodology is demonstrated with a different gene.
- •
High correlation with RT-qPCR demonstrated its utility for splicing event monitoring.