Evaluation of two recombinant Leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis
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- 作者：Eduardo Antonio Ferraz Coelhoa ; eduardoferrazcoelho@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Lourena Emanuele Costaa ; 1 ; Daniela Pagliara Lagea ; 1 ; Ví ; vian Tamietti Martinsb ; 1 ; Esther Gardec ; 1 ; Nathá ; lia Cristina de Jesus Pereiraa ; 1 ; Eliane Gonç ; alves Paiva Lopesd ; Luiz Felipe Nunes Menezes Borgese ; Mariana Costa Duartef ; Daniel Menezes-Souzaf ; Danielle Ferreira de Magalhã ; es-Soaresd ; Miguel Angel Chá ; vez-Fumagallia ; Manuel Sotoc ; Carlos Alberto Pereira Tavaresb
- 关键词：Serodiagnosis ; Recombinant proteins ; Leishmania infantum ; Canine visceral leishmaniasis ; Human tegumentary leishmaniasis ; Leishmania braziliensis
- 刊名：Veterinary Parasitology
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：215
- 期：Complete
- 页码：63-71
- 全文大小：1175 K
文摘
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Antigenic proteins were selected in a proteomic approach in Leishmania infantum.
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Two of them, HRF and CcOx, were cloned, expressed and purified.
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The recombinant proteins were evaluated for the serodiagnosis of leishmaniasis.
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Both proteins showed sensitivity and specificity values of 100% in the ELISA assays.
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The proteins could be used for the serodiagnosis of canine and human leishmaniasis