Dried extracts of Encholirium spectabile (Bromeliaceae) present antioxidant and photoprotective activities in聽vitro

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• 作者：Raimundo Gon莽alves de Oliveira Jr. ; Grasielly Rocha Souza ; Am ; a Leite Guimar茫es ; Ana Paula de Oliveira ; Am ; a Caroline Silva Morais ; Edig锚nia Cavalcante da Cruz Ara煤jo ; Xirley Pereira Nunes ; Jackson Roberto Guedes da Silva Almeida
• 关键词：Antioxidant properties ; Bromeliaceae ; Encholirium spectabile ; Medicinal plants ; Photoprotection
• 刊名：Journal of Young Pharmacists
• 出版年：September, 2013
• 年：2013
• 卷：5
• 期：3
• 页码：102-105
• 全文大小：295 K

文摘

The antioxidant and photoprotective activities of dried extracts from the leaves of Encholirium spectabile were investigated. It was also evaluated the total phenolic and flavonoid contents by the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using of 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and 尾-carotene-linoleic acid bleaching and compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) used as reference compounds. The photoprotective effect was evaluated by the spectrophotometric method. The most significant total phenolic and flavonoid contents was of 188.50聽卤聽27.50聽mg of gallic acid equivalent/g and 129.70聽卤聽4.59聽mg of catechin equivalent/g, respectively, for chloroform fraction (Es-CHCl₃). The Es-CHCl₃ also presented the best antioxidant activity (IC₅₀ 25.35聽卤聽4.35聽渭g/ml) for DPPH scavenging. The ethanol extract (Es-EtOH), Es-CHCl₃ and the fraction ethyl acetate (Es-AcOEt) showed characteristic absorption bands in regions UVB and UVA in a concentration-dependent manner. Es-CHCl₃ presented the highest sun protection factor SPF (8.89聽卤聽2.11). It shows the possibility to use this extract as sunscreen in pharmaceutical preparations.