• 作者：P. May-Panloup ; V. Ferr¨¦-L¡¯H?tellier ; C. Morini¨¨re ; C. Marcaillou ; S. Lemerle ; A. Coutolleau ; P. Reynier ; P. Descamps ; P. Guardiola
• 关键词：Corona radiata ; Cellules folliculeuses ; Expression gé ; nique ; Ré ; serve ovarienne
• 刊名：Gynecology Obstetrique & Fertilite
• 出版年：2012
• 出版时间：September, 2012
• 年：2012
• 卷：40
• 期：9
• 页码：500-506
• 全文大小：824 K

ss=""h4"">Objectives

Diminished ovarian reserve (DOR) is one of the causes of infertility. In this prospective study, gene expression profiling (GEP) of corona radiata cells (CRC) was performed to identify genes deregulated in DOR patients.

ss=""h4"">Patients and methods

Microarray-based GEP of CRC isolated from eight women undergoing IVF was performed to identify genes differentially expressed between patients with normal ovarian reserve and DOR patients. Microfluidic-based quantitative RT-PCR assay were used to validate selected transcripts on 40?independent patients.

ss=""h4"">Results

Forty-eight transcripts were differentially expressed, including CXXC5?and FOXC1?down regulated in DOR, as well as CTGF, FSTL3, PTGS2?and SOCS2?up regulated in DOR. According to these transcripts, two DOR patients'subgroups (DOR Gr1?and Gr2) were identified. In DOR Gr2?patients, CITED2, CTGF, GAS-1, IRS2, PTGS2, SOCS2, VCAN were expressed at significantly higher levels, and CXXC5, FOXC1, GBP2?and ZMIZ1?at significantly lower level. Eleven of those genes are transcriptional targets of Estrogens and higher baseline oestradiol levels were observed in DOR Gr2?patients (P < 0.006).

ss=""h4"">Discussion and conclusion

Twelve genes deregulated in CRC of DOR patients were identified, which could be involved in DOR pathogenesis. The distinction of a particular subgroup of DOR patients suggests the possibility of deregulation of estrogen response genes.