Strategies for over-expression and purification of recombinant full length STAT5B in Escherichia coli
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- 作者：Elvin D. de Araujoa ; b ; Mulu Geletua ; b ; Patrick T. Gunninga ; b ; patrick.gunning@utoronto.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Recombinant Signal Transducer and Activator of Transcription 5B (STAT5B) expression ; Peptide fluorescence polarization ; Thermal shift assay ; Western blotting ; SYPRO Orange ; Osmolyte
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：1-8
- 全文大小：623 K
文摘
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Recombinant full length STAT5B was over-expressed and purified in Escherichia coli.
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Thermal shift assays identified buffer conditions for optimal stability of STAT5B.
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Fluorescence polarization assays indicate purified STAT5B is functional.