- 作者：Brigitta L¨¢szl¨® ; J¨®zsef K¨®nya ; Eszter D ; ¨¢r ; Judit De¨¢k ; ¨¢gnes Farkas ; Jim Gray ; G¨¢bor Gr¨®sz ; Miren Iturriza-Gomara ; Ferenc Jakab ; ¨¢gnes Juh¨¢sz ; P¨¦ter Kisfali ; Julianna Kov¨¢cs ; Gy?rgy Lengyel ; Vito Martella ; B¨¦la Melegh ; J¨²lia M¨¦sz¨¢ros ; P¨¦ter Moln¨¢r ; Zolt¨¢n Ny¨²l ; Hajnalka Papp ; L¨¢szl¨® P¨¢tri ; et al.
- 关键词：Gastroenteritis ; Molecular epidemiology ; VP7 ; VP4 ; Genotype
- 刊名：Journal of Clinical Virology
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：55
- 期：2
- 页码：140-146
- 全文大小：606 K
Background
The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains.Objectives
In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against.
Study design
The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5 years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed.
Results
In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87 % ) strains, followed by G4P[8] (23.4 % ), G2P[4] (14.75 % ) and G9P[8] (6.81 % ) genotypes. Uncommon strains were identified in a low percentage of samples (4.12 % ). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9 % ; VP4, up to 98.5 % ) although their vaccine origin was unlikely.
Conclusions
Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.