6-Azido d-galactose transfer to N-acetyl-d-glucosamine derivative using commercially available β-1,4-galactosyltransferase
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- 作者：Michaë ; l Bosco ; Sophie Le Gall ; Christophe Rihouey ; Samuel Couve-Bonnaire ; Muriel Bardor ; Patrice Lerouge ; Xavier Pannecoucke
- 关键词：Endocrine disrupting compounds ; Pharmaceutically active compounds ; Nanofiltration ; Reverse osmosis ; Fouling
- 刊名：Tetrahedron Letters
- 出版年：2008
- 出版时间：31 March 2008
- 年：2008
- 卷：49
- 期：14
- 页码：2294-2297
- 全文大小：148 K
文摘
A new strategy to tag glycoproteins carrying terminal GlcNAc was developed using commercially available bovine β-1,4-galactosyltransferase (GalT) and UDP-6-azidogalactose. The azide function was then allowed to react via a biotinylated Staudinger–Bertozzi probe demonstrating the usefulness of such a procedure to tag any glycoprotein possessing a N-acetylglucosamine terminal residue from any type of cell lysate.