Detection of cytomegalovirus DNA on dried blood spots collected from infants infected with HIV: An in-house method adaptable in resource-limited settings

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• 作者：Marianne Leruez-Ville ; Sopeak Ngin ; Tiffany Guilleminot ; Anfumbom Kfutwah ; S ; rine Moussa ; Ton Tran ; Eric Nerrienet
• 关键词：DBS ; CMV ; HIV ; Resource-limited countries
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：193
• 期：2
• 页码：503-507
• 全文大小：522 K

文摘

In countries with limited resources, infants infected with HIV are highly exposed to CMV co-infection which probably represents a major risk factor for disease progression in this population. This study aimed to evaluate the performance of a low cost CMV DNA extraction method from DBS and the feasibility of its implementation in laboratories of 4 countries with limited resources. DNA was extracted from DBS with a cationic resin (chelex 100) and amplified with an ¡°in house¡± real time CMV PCR. Dilutions of a quantified whole blood sample were spotted on paper to evaluate the 95 % detection limit. A DBS quality control panel was analyzed in all laboratories. CMV PCR was compared between DBS and liquid whole blood (gold standard) in 2 populations: 418 transplanted patients and 59 infants infected with HIV (median age of 2 months). The CMV PCR 95 % detection limit in DBS was 3.87 log₁₀ copies/mL. Its positive and negative predictive values for CMV diagnosis in infants infected with HIV were 100 % and 87.5 % respectively. Quality control panels gave consistent qualitative results in all laboratories. This assay had high predictive values for CMV diagnosis in infants infected with HIV and its implementation in resource-limited countries with limited resources is feasible.