The effects of transdermal permeation enhancers on thermotropic phase behaviour of a stratum corneum lipid model

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• 作者：J. Zbytovská ; K. Vá ; vrová ; M.A. Kiselev ; P. Lessieur ; S. Wartewig ; R.H.H. Neubert
• 关键词：Stratum corneum lipids ; Small-angle X-ray diffraction ; Thermotropic phase behaviour ; Urea ; Oleic acid ; Ceramide analogue
• 刊名：Colloids and Surfaces A: Physicochemical and Engineering Aspects
• 出版年：2009
• 出版时间：5 November 2009
• 年：2009
• 卷：351
• 期：1-3
• 页码：30-37
• 全文大小：536 K

文摘

This study evaluates the interactions of a model stratum corneum (SC) lipid system based on ceramide AP (N-(2-hydroxystearoyl)phytosphingosine) with three selected permeation enhancers including urea, oleic acid (OA), and N-lauroylglycine lauryl ester (12G12) using temperature-dependent small-angle X-ray diffraction measurements. As a first step, the thermotropic phase behaviour of the control SC lipid membrane, i.e. without enhancer, was characterized. The system shows two separated phases at 32 °C, which mix into another phase at around 45 °C. This phase is stable till 70 °C when the repeat distance starts to decrease. After cooling, only one phase is visible which shows two phase transitions at 45 and 70 °C again. Based on these results, the effects of the permeation enhancers were studied. The permeation enhancers influence the phase behaviour of the system. Urea and 12G12 cause a concentration-dependent shift of the phase transition temperatures while OA induces a phase separation. The results from this simple model system may provide basis for studies on more complex systems or real SC.