Benefits of active oxygenation during hypothermic machine perfusion of kidneys in a preclinical model of deceased after cardiac death donors

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• 作者：Rapha?l Thuillier ; Geraldine Allain ; Olivier Celhay ; William Hebrard ; Benoit Barrou ; Lionel Badet ; Henri Leuvenink ; Thierry Hauet
• 关键词：Kidney transplantation ; DCD ; Machine perfusion ; Oxygen ; Marginal donors ; Preclinical
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：184
• 期：2
• 页码：1174-1181
• 全文大小：1056 K

文摘

Background

Deceased after cardiac death donors (DCDs) represent a valuable source of organs; however, preventing poor outcome is difficult, even with the use of machine perfusion (MP). It is of paramount importance to improve this method. We proposed to evaluate the benefits of active oxygenation during kidney graft hypothermic MP using a novel perfusion machine: Kidney Assist (KA).

Methods

We used a pig model of DCD transplantation in Large White pigs. Cold preservation was performed by conventional non-oxygenated MP (KAnoO₂) or oxygenated MP (KA).

Results

In the first 2?wk post-transplant, KA grafts displayed a lower serum creatinine peak and a faster return to normal levels compared with KAnoO₂ animals, translating into a smaller area under the curve. Urinary neutrophil gelatinase-associated lipocalin levels and serum aspartate amino transferase levels were lower in KA animals compared with the non-oxygenated group. These correlated with better chronic function. Longer follow-up of the animals (3?mo) permitted evaluation of chronic outcome lesions. Interstitial fibrosis was reduced in the KA group, and these kidneys also displayed significantly lower levels of vimentin staining. Further histologic investigation also showed a trend toward decreased chronic inflammation in kidneys preserved with oxygen.

Conclusions

This new MP system is efficient in preserving DCD kidneys, greatly enhancing the capacity of the graft to withstand preservation stress and improving outcome. Oxygen delivery during preservation is thus valuable for highly damaged organs and offers an important therapeutic tool for transplant teams faced with decreased quality of donor organs.