Syngas fermentation to biofuels: Effects of hydrogen partial pressure on hydrogenase efficiency

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• 作者：Bradley E. Skidmore ; Ryan A. Baker ; Dila R. Banjade ; Jason M. Bray ; Douglas R. Tree ; Randy S. Lewis ; ^{randy.lewis@byu.edu}
• 关键词：Syngas ; Fermentation ; Biofuels ; Hydrogenase ; Ethanol ; Clostridium
• 刊名：Biomass and Bioenergy
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：55
• 期：Complete
• 页码：156-162
• 全文大小：536 K

文摘

Producing biofuels from gasified biomass (synthesis gas) via microbial fermentation is currently being pursued as one alternative in biofuels development. In synthesis gas fermentation, reducing equivalents from H₂ oxidation via hydrogenase is important towards directing more carbon towards product formation. In this work, kinetic studies of H₂ utilization via the Clostridium P11 hydrogenase enzyme were performed to determine the most appropriate model to predict hydrogenase activity as a function of H₂ partial pressure. An important aspect of this work included the proper analysis of electron acceptors used in the kinetic studies. The model parameter governing the effect of H₂ partial pressure on activity was ¡«30?kPa (absolute), independent of the type and concentration of electron acceptor. The value indicates that H₂ partial pressures typically associated with syngas fermentation will result in compromised efficiency of the hydrogenase activity.