LongR3 enhances Fc-fusion protein N-linked glycosylation while improving protein productivity in an industrial CHO cell line
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- 作者：Yueming Qian ; yueming.qian@bms.com ; Amanda M. Lewis ; Sarah M. Sidnam ; Alison Bergeron ; Nicholas R. Abu-Absi ; Nisha Vaidyanathan ; Adam Deresienski ; Nan-Xin Qian ; Michael C. Borys ; Zheng Jian Li
- 关键词：Cell culture ; Chinese Hamster Ovary (CHO) ; Glycosylation ; PCR array ; Protein production ; Sialic acid ; Neu2
- 刊名：Process Biochemistry
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：53
- 期：Complete
- 页码：201-209
- 全文大小：2046 K
- 卷排序：53
文摘
Insulin-like growth factor analog LongR3 increases sialic acid content of Fc-fusion protein. LongR3 down-regulates sialidase gene expression. Decreased extracellular sialidase prevents recombinant glycoprotein desialylation.