LC-MS/MS analysis and pharmacokinetic study on five bioactive constituents of Tanreqing injection in rats

详细信息    查看全文

• 作者：Feng ZHANG^a ; Liang SUN^a ; ^b ; ^c ; ^# ; Shou-Hong GAO^a ; Wan-Sheng CHEN^a ; ^{chenwansheng@smmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{chenwanshengsmmu@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Yi-Feng CHAI^b ; ^{yfchai@smmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tanreqing Injection ; Flavones ; Phenolic acids ; Bile acids ; Pharmacokinetics
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：14
• 期：10
• 页码：769-775
• 全文大小：1156 K

文摘

Tanreqing injection (TRQ), a well-known traditional Chinese medicine formula, is commonly used to treat respiratory diseases. In the present study, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determinate the plasma contents of 5 major constituents of TRQ, including chlorogenic acid (CHA), caffeic acid (CFA), baicalin (BA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in rats after intravenous administration of TRQ. Chromatographic separation was performed on an Agilent Zorbax SB-C₁₈ column (3.5 μm, 100 mm × 2.1 mm), with acetonitrile and 0.1% aqueous formic acid as mobile phase at a flow rate of 0.3 mL·min⁻¹. The calibration curves were linear over the ranges of 27.0–13 333.0 ng·mL⁻¹ for CFA, 30.0–14 933.0 ng·mL⁻¹ for CHA, 50.0–50 333.0 ng·mL⁻¹ for BA, 550.0–55 000.0 ng·mL⁻¹ for UDCA, and 480.0–48 000.0 ng·mL⁻¹ for CDCA, respectively. Intra- and inter-day precisions (relative standard deviations, RSDs) were from 3.11% to 14.08%. The extraction recoveries were greater than 71% and accuracy (relative recovery) was from 89% to 137% for all analytes, except endogenous bile acids. This validated method was successfully applied to the first pharmacokinetic study of CFA, CHA, BA, UDCA and CDCA in rat plasma after intravenous administration of TRQ.