文摘
Pneumococcal iron acquisition ATP binding cassette is abbreviated to PiaABC. The cell-surface lipoprotein PiaA is a key component of PiaABC. Trp63, Trp158 and Phe255, in the metal binding site of PiaA, were mutated. Thermodynamic and kinetic study on the ferrichrome binding ability was performed. Trp158 is a crucial residue for structure stability and ferrichrome binding of PiaA.