Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo

详细信息    查看全文

• 作者：Chia-Chen Pi ; Ching-Liang Chu ; Chu-Ying Lu ; Yu-Jing Zhuang ; Cheng-Li Wang ; Yao-Hsuan Yu ; Hui-Yi Wang ; Chih-Chung Lin ; Chun-Jen Chen
• 关键词：Vaccine ; Adjuvant ; Ganoderma formosanum ; Polysaccharides ; Th1 response ; CTL response
• 刊名：Vaccine
• 出版年：9 January, 2014
• 年：2014
• 卷：32
• 期：3
• 页码：401-408
• 全文大小：1449 K

文摘

The fungus of Ganoderma is a basidiomycete that possesses a variety of pharmacological effects and has been used in traditional Asian medicine for centuries. Ganoderma formosanum is a native Ganoderma species isolated in Taiwan, and we have previously demonstrated that PS-F2, a polysaccharide fraction purified from the submerged culture broth of G. formosanum, exhibits immunostimulatory properties in macrophages. In this study, we further characterized the adjuvant functions of PS-F2. In vitro, PS-F2 stimulated dendritic cells (DCs) to produce proinflammatory cytokines, including TNF-伪, interleukin (IL)-6, and IL-12/IL-23 p40. PS-F2 also stimulated DCs to express the maturation markers CD40, CD80, CD86, and MHC class II. In a murine splenocyte culture, PS-F2 treatment resulted in elevated expression of T-bet and interferon (IFN)-纬 in T lymphocytes. When used as an adjuvant in vivo with the ovalbumin (OVA) antigen, PS-F2 stimulated OVA-specific antibody production and primed IFN-纬 production in OVA-specific T lymphocytes. PS-F2-adjuvated immunization also induced OVA-specific CTLs, which protected mice from a challenge with tumor cells expressing OVA. Collectively, our data show that PS-F2 functions as an adjuvant capable of inducing a Th1-polarized adaptive immune response, which would be useful in vaccines against viruses and tumors.