Comparison of Second-Phase Insulin Secretion Derived from Standard and Modified Low-Dose Graded Glucose Infusion Tests

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• 作者：Jiunn-Diann Lin ; MD^a ; Chung-Ze Wu ; MD ; PhD^a ; Wen-Hao Tang ; MD^b ; Feng-Chih Kuo ; MD^b ; Dee Pei ; MD^c ; Yao-Jen Liang ; PhD^d ; Yen-Lin Chen ; MD^e ; ^{anthonypatho@gmail.com}
• 关键词：low-dose graded glucose infusion test ; modified low-dose graded glucose infusion test ; second-phase insulin secretion
• 刊名：Canadian Journal of Diabetes
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：40
• 期：6
• 页码：529-534
• 全文大小：508 K
• 卷排序：40

文摘

Insulin secretion has 2 phases, first-phase and second-phase insulin secretion (SPIS). The purpose of our study was to validate the SPIS derived from the modified low-dose graded glucose infusion test (MLDGGI) by using the standard SPIS derived from the low-dose graded glucose infusion test (LDGGI).MethodsFourteen participants were enrolled. The participants underwent the LDGGI for 200 minutes and MLDGGI for 60 minutes. The LDGGI was administered using the standard Polonsky method, which entails 5 40-minute stages of glucose infusion (1, 4, 8, 6 and 24 mg/kg/min). The slopes of the insulin levels vs. the glucose levels obtained during the test were regarded as SPIS (SPIS-I). Furthermore, deconvolution was performed, and slopes of the insulin secretion rate (ISR) against glucose levels during the test (SPIS-D) were obtained and regarded as the gold standard SPIS. The MLDGGI was administered with 3 20-minute stages of glucose infusion (2, 8 and 24 mg/kg/min) for 20 minutes each. The slopes of the insulin levels vs. the glucose levels obtained during the test were regarded as SPIS (MSPIS-I). In addition, deconvolution was performed, and the slopes of the ISR against glucose levels during the test (MSPIS-D) were obtained.ResultsSignificant correlations were observed between SPIS-I and SPIS-D (r=0.843; p<0.001) and between MSPIS and SPIS-D (r=0.786; p<0.001).ConclusionsSPIS could be determined simply by using the LDGGI without deconvolution. Moreover, MLDGGI was proved to be useful in estimating SPIS easily on the basis of insulin levels.