Two new cytotoxic triterpenoid saponins from the roots of Clematis argentilucida

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• 作者：Wenli Hai^a ; Hua Cheng^b ; Mei Zhao^c ; Yi Wang^a ; Liangjian Hong^a ; Haifeng Tang^a ; ^{tanghaifeng71@163.com} ; Xiangrong Tian^a ; ^d ; ^{tianxiangrong@163.com}
• 关键词：Ranunculacea ; Clematis argentilucida ; Triterpenoid saponin ; Cytotoxicity
• 刊名：Fitoterapia
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：83
• 期：4
• 页码：759-764
• 全文大小：357 K

文摘

Bioassay-guided fractionation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of two new triterpenoid saponins along with a known one, cussonside B (3). By extensive spectral analysis and chemical evidences, the structures of the two new saponins were determined to be 3¦Â-O-[¦Â-d-ribopyranosyl-(1 ?#xA0;3)-¦Á-l-rhamnopyranosyl-(1 ?#xA0;2)-¦Á-l-arabinopyranosyl] hederagenin-11,13-dien-28-oic acid (1) and 3¦Â-O-{¦Â-d-ribopyranosyl-(1 ?#xA0;3)-¦Á-l-rhamnopyranosyl-(1 ?#xA0;2)-[¦Â-d-glucopyranosyl-(1 ?#xA0;4)]-¦Â-d-xylopyranosyl} oleanolic acid (2), respectively. Saponin 1 is the first example of triterpenoid saponins with two double bonds located at C-11 and C-13 in the aglycone from the genus Clematis. The two new saponins exhibited significant cytotoxicity against human leukemia HL-60 cell lines, human hepatocellular carcinoma Hep-G2 cell lines and human glioblastoma U251MG cell lines with a range of IC₅₀ values from 2.74 to 25.40 ¦ÌM, while 3 showed inactivity against all of the three cancer cell lines.