Catalpol stimulates VEGF production via the JAK2/STAT3 pathway to improve angiogenesis in rats’ stroke model

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• 作者：Wan Dong^a ; Yang Xian^b ; Wang Yuan^c ; ^d ; ^e ; Zhu Huifeng^c ; ^d ; ^e ; ^{zhfbsci@126.com" class="auth_mail" title="E-mail the corresponding author} ; Wang Tao^c ; ^d ; ^e ; Liu Zhiqiang^f ; Feng Shan^c ; ^d ; ^e ; Fu Ya^g ; Wang Hongli^c ; ^d ; ^e ; Wang Jinghuan^c ; ^d ; ^e ; Qin Lei^c ; ^d ; ^e ; Zou Li^c ; ^d ; ^e ; Qi Hongyi^c ; ^d ; ^e
• 关键词：Catalpol ; VEGF ; JAK2/STAT3 ; Angiogenesis
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：169-179
• 全文大小：4012 K

文摘

Catalpol is the main active component of the radix from Rehmannia glutinosa Libosch, which has pleiotropic protective effects in neurodegenerative diseases, ischemic stroke, metabolic disorders and others

Aim

Catalpol has been shown to have neuroprotective, neurorepair, and angiogenesis effects following ischemic brain injury. However, its molecular mechanisms are still poorly understood. In previous studies, the JAK2/STAT3 signaling pathway was found to play a role in neuroprotection and angiogenesis. This study investigated the role of catalpol in stimulating angiogenesis via the JAK2/STAT3 pathway after permanent focal cerebral ischemia (pMCAO).

Methods

Rats were subjected to right middle cerebral artery occlusion through electrocoagulation and were treated with catalpol (5 mg/kg), AG490 was also used to inhibit STAT3 phosphorylation (pSTAT3).

Results

Following stroke, Catalpol improved the neuroethology deficit, increased the cerebral blood flow (CBF) of infarcted brain and upregulated EPO and EPOR. AG490 suppressed the phosphorylation of signal transducer and activator of transcription 3 (STAT3), ultimately inhibited VEGF mRNA expression, which reduced VEGF protein expression and inhibited stroke-induced angiogenesis. However, Catalpol enhanced stroke-induced STAT3 activation and subsequently restored STAT3 activity through the recovery of STAT3 binding to VEGF. Moreover, Catalpol reversed the effect of AG490 on STAT3 activation and nuclear translocation, restored the transcriptional activity of the VEGF promoter by recruiting STAT3 to the VEGF promoter, improved VEGF mRNA and protein expression, increased angiogenesis, reduced the difference in CBF between the infarcted and intact brain and ameliorated the neuroethology behaviors after stroke.

Conclusion

Catalpol affects neuroprotection and angiogenesis via the JAK2/STAT3 signaling pathway, which is mediated by STAT3 activation and VEGF expression. Catalpol may be used as a potential therapeutic drug for stroke.