The effect of licochalcone A on cell-aggregates ECM secretion and osteogenic differentiation during bone formation in metaphyseal defects in ovariectomized rats

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• 作者：Fenqing Shang ; Leiguo Ming ; Zhifei Zhou ; Yang Yu ; Jinlong Sun ; Yin Ding ; Yan Jin
• 关键词：Licochalcone A ; Mesenchymal stem cells ; Cell aggregate ; Bone regeneration ; Osteoporosis
• 刊名：Biomaterials
• 出版年：March, 2014
• 年：2014
• 卷：35
• 期：9
• 页码：2789-2797
• 全文大小：3572 K

文摘

Treatment of weight-bearing bones fractures with defects is critical for patients with osteoporosis's rehabilitation. Although various tissue engineering methods were reported, the best treating strategy for tissue engineering remains to be identified as the limitation of enhancing the ability of the osteogenetic differentiation potential of seed cell is one of the cardinal issues to be solved. The objective of this study is to investigate the feasibility of applying licochalcone-A (L-A) and bone marrow mesenchymal stem cells (BMSC)-aggregate in bone repairing tissue engineering and further study the biological effects of L-A on the cell aggregate formation and osteogenic properties. 80 female Sprague聽Dawley rats underwent bilateral ovariectomy were made with a 3.5聽mm femurs bone defects without any fixation. These rats were then randomly assigned to five different treatment groups: (1) empty defect (n聽=聽16), (2) CA-LA (n聽=聽16), (3) CA-N (n聽=聽16), (4) CA-L (n聽=聽16), (5) CA-S (n聽=聽16) and 16 female SD rats were treated as a control. Data showed that L-A administrated cell aggregate had a stronger osteogenic differentiation and mineralized formation potential than non-administrated group both in聽vitro and in聽vivo. For in聽vitro study, L-A administrated group had a more significant expression of ECM, osteogenic associated maker in addition with more mineralized area and higher ALP activity compared with the control group. For in聽vivo study, 3D reconstruction of micro-CT, HE staining and bone strength results showed that newly formed bone in groups administrated by L-A was significant higher than that in Sham group at 2, 4, 8 and 12 weeks after transplantation, especially for groups which was systematically injected with L-A at 8 weeks. Results of our study demonstrated that LA could positively affect cell behavior in cell-aggregate engineering and could be a promising strategy in treating osteoporotic weight-bearing bones fractures with defects.