The two component adjuvant IC31^® potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice

详细信息    查看全文

• 作者：Lidice Bernardo^a ; Alequis Pavó ; n^a ; Lisset Hermida^b ; Lá ; zaro Gil^b ; Iris Valdé ; s^b ; Sheila Cabezas^a ; Ramó ; n Linares^a ; Mayling Alvarez^a ; Ricardo Silva^b ; Gerardo Guillé ; n^b ; Eszter Nagy^c ; Petra Schlick^c ; Marí ; a G. Guzmá ; n^a ; ^{lupe@ipk.sld.cu"" rel=""nofollow}
• 关键词：Dengue ; Domain III ; IC31^® ; adjuvant
• 刊名：Vaccine
• 出版年：2011
• 出版时间：6 June 2011
• 年：2011
• 卷：29
• 期：25
• 页码：4256-4263
• 全文大小：710 K

文摘

Here we evaluated the suitability of the synthetic adjuvant IC31^® to potentiate the protective capacity of PD5 protein (domain III of the envelope protein of dengue 2 virus fused to the carrier protein P64k). Unlike Alum, PD5 mixed with IC31^® induced complete protection against virus challenge in mice and increased IFN-γ secretion after in vitro re-stimulation. The induced antibody response was highly specific to the homologous serotype and showed both IgG1 and IgG2a subtypes. IC31^® is a promising adjuvant for PD5 recombinant protein based vaccination against dengue. Future work should address the suitability of PD5/IC31^® formulations in non-human primate models.