Signal transduction pathways involved in mechanical regulation of HB-GAM expression in osteoblastic cells

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• 作者：Astrid Liedert ; Daniela Kaspar ; Lutz Claes and Anita Ignatius
• 关键词：Heparin-Binding Growth-Associated Molecule ; Osteoblastic cells ; Mechanical loading ; Mechanotransduction ; Signal transduction ; PKC ; PKA ; MAPK
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：21 April 2006
• 年：2006
• 卷：342
• 期：4
• 页码：1070-1076
• 全文大小：227 K

文摘

Protein kinase C (PKC), protein kinase A (PKA), prostaglandin synthesis, and various mitogen-activated protein kinases (MAPKs) have been reported to be activated in bone cells by mechanical loading. We studied the involvement of these signal transduction pathways in the downregulation of HB-GAM expression in osteoblastic cells after cyclic stretching. Specific antagonists and agonists of these signal transduction pathways were added to cells before loading and to non-loaded control cells. Quantitative RT-PCR was used to evaluate gene expression. The data demonstrated that the extracellular signal-regulated kinase (ERK) 1/2 pathway, PKC, PKA, p38, and c-Jun N-terminal kinase MAPK participated in the mechanical downregulation of HB-GAM expression, whereas prostaglandin synthesis did not seem to be involved.