Mechanosensitive promoter region in the human HB-GAM gene

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• 作者：Astrid Liedert ; Moustapha Kassem ; LutzClaes ; Anita Ignatius
• 关键词：Heparin-binding growth-associated molecule ; Mesenchymal stem cell ; Mechanical loading ; Mechanosensitivity ; Promoter ; Transcription
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：18 September 2009
• 年：2009
• 卷：387
• 期：2
• 页码：289-293
• 全文大小：244 K

文摘

Mechanical loading is essential for maintaining bone mass in the adult skeleton. However, the underlying process of the transfer of the physical stimulus into a biochemical response, which is termed mechanotransduction is poorly understood. Mechanotransduction results in the modulation of gene expression through specific transcription factor binding sites in the promoter region of mechanosensitive genes. In the present study, we demonstrate that the expression of HB-GAM, which is known to have stimulating effects on osteogenic differentiation, is rapidly induced by mechanical loading in hMSC-TERT4 cells. Analysis of the human HB-GAM gene upstream regulatory region with luciferase reporter gene assays revealed that the upregulation of HB-GAM expression occurred at the transcriptional level and was mainly dependent on the HB-GAM promoter region most upstream containing three potential AP-1 binding motifs.