Exploring the effect and mechanism of Hibiscus sabdariffa on urinary tract infection and experimental renal inflammation

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• 作者：Shun-Ting Chou^a ; ¹ ; Hsin-Yi Lo^a ; ¹ ; Chia-Cheng Li^a ; Lu-Chen Cheng^a ; ^b ; Pei-Chi Chou^c ; Yu-Chen Lee^d ; ^e ; Tin-Yun Ho^a ; ^f ; ^{tyh@mail.cmu.edu.tw} ; Chien-Yun Hsiang^g ; ^{cyhsiang@mail.cmu.edu.tw}
• 关键词：Ccl12 ; chemokine (C-C motif) ligand 12 ; Cox2 ; cyclooxygenase 2 ; Cxcl2 ; chemokine (C-X-C motif) ligand 2 ; D3S ; delphinidin-3-sambubioside ; ELISA ; enzyme-linked immunosorbent assay ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; HPLC ; high-performance liquid chromatography ; IHC ; immunohistochemical staining ; IL-1β ; interleukin-1β ; iNOS ; inducible nitric oxide ; LPS ; lipopolysaccharide ; MTT ; methylthiazolyldiphenyl-tetrazolium bromide ; MAPK ; mitogen-activated protein kinase ; MCP-1 ; monocyte chemoa
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：24 December 2016
• 年：2016
• 卷：194
• 期：Complete
• 页码：617-625
• 全文大小：1157 K
• 卷排序：194

文摘

Hibiscus sabdariffa Linn., also known as roselle, is used in folk medicine as an anti-inflammatory agent. Urinary tract infection (UTI) is a common problem in long-term care facilities. However, effects of roselle on UTI and renal inflammation remained to be analyzed.AimHere we surveyed the effect of roselle drink on the prevention of UTI in long-term care facilities and analyzed the anti-inflammatory potential of roselle on lipopolysaccharide (LPS)-induced renal inflammation in mice.Materials and methodsSurvey questionnaires and clinical observation were applied to evaluate the use of roselle and the incidence of UTI in long-term care facilities. Mice were administrated roselle orally for 7 consecutive days and then challenged with LPS. Anti-renal inflammatory effects of roselle were analyzed by microarray and immunohistochemical staining.ResultsClinical observation showed that taking roselle drink in residents with urinary catheters reduced the incidence of UTI in long-term care facilities. Renal inflammation is a key event of UTI. Roselle suppressed LPS-induced nuclear factor-κB (NF-κB) activation in cells and LPS-induced interleukin-1β production in mice a dose-dependent manner. Immunohistochemical staining showed that roselle inhibited LPS-induced NF-κB activation and inflammatory cell infiltration in kidney. Gene expression profiling further showed that roselle suppressed the expression of pro-inflammatory cytokine genes and enzyme genes involved in the production of prostaglandin and nitric oxide. In addition, NF-κB was the main transcription factor involved in the regulation of roselle-regulated gene expression in kidney.ConclusionsThis is the first report applying clinical observation-guided transcriptomic study to explore the application and mechanism of roselle on UTI. Our findings suggested that roselle drink ameliorated LPS-induced renal inflammation via downregulation of cytokine network, pro-inflammatory product production, and NF-κB pathway. Moreover, this report suggested the potential benefit of roselle drink on UTI.