The binding, synergistic and structural characteristics of BsEXLX1 for loosening the main components of lignocellulose: Lignin, xylan, and cellulose
详细信息 查看全文
- 作者：Qun Wanga ; Liang Chenb ; Hui Linc ; Daobing Yud ; Qi Shena ; Li Wane ; Yuhua Zhaoa ; microbeapply@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lignocellulose loosening ; BsEXLX1 ; Site-directed mutagenesis ; Synergistic activity ; Homologous modeling
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：92
- 期：Complete
- 页码：67-75
- 全文大小：2019 K
文摘
- •
BsEXLX1 was found to preferentially bind to xylan when loosening lignocellulose.
- •
The binding between heterogenous expressed BsEXLX1 and substrates did not saturate.
- •
Residue-125, 126, and 171 of BsEXLX1 were less important for the binding to xylan.
- •
Residue-125 and 171 play more important role in structural maintenance of BsEXLX1.
- •
The synergistic activity of BsEXLX1 is not correlated with its specific binding.