Ultrasensitive electrochemical biosensing strategy for microRNA-21 detection based on homogeneous target-initiated transcription amplification

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• 作者：Hao Wang^a ; ^b ; ¹ ; Zhong Zuo^c ; ¹ ; Lihong Ren^a ; ^b ; Rui Yuan^d ; Qifu Li^b ; Shijia Ding^a ; ^d ; ^{dingshijia@163.com} ; Rong Luo^a ; ^b ; ^{luorongy@163.com}
• 关键词：MiRNA-21 ; Homogeneous transcription amplification ; Enzymatic amplification ; Electrochemical biosensing ; HepG2
• 刊名：Journal of Electroanalytical Chemistry
• 出版年：2016
• 出版时间：15 December 2016
• 年：2016
• 卷：783
• 期：Complete
• 页码：22-27
• 全文大小：812 K
• 卷排序：783

文摘

This work provided a simple, ultrasensitive and low-cost electrochemical biosensing strategy for miRNA-21 detection. T7 RNA polymerase based homogeneous transcription and enzymatic amplification were used for signal amplification. This method was stable, specificity and sensitivity in miRNA-21 detection. This method was successfully applied to detect miRNA-21 in total RNA extracted from HepG2 cells.