Preparation and characterization of carboxymethylated carrageenan modified with collagen peptides
详细信息 查看全文
- 作者：Lihong Fan ; lihongfan2000@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Jun Tong ; Chang Tang ; Huan Wu ; Min Peng ; Jiayan Yi
- 关键词：Carboxymethyl κ-carrageenan collagen peptide ; Cell viability ; Wound healing
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：790-797
- 全文大小：1499 K
文摘
The preparation of carboxymethyl κ-carrageenan collagen peptide (CMKC-COP) was via an imide-bond forming reaction between carboxyl groups in carboxymethyl κ-carrageenan (CMKC) and amino groups in collagen peptide in the presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy sulfosuccinimide (NHS). CMKC-COP products were verified with infrared spectroscopy (FT-IR). The results of degree of substitution (DS) of CMKC-COP was presented, which are depended on reaction time, molar ratio of collagen peptide to carboxymethyl κ-carrageenan and reaction temperature. The optimal reaction conditions were studied by means of single factor experiment. Also MTT assay was applied to evaluate the effects of CMKC-COP on proliferation of chick embryo fibroblasts. The animal experiment results indicated that the wound covered with CMKC-COP were completely filled with new epithelium within 2 weeks without any significant adverse side reactions. Therefore, the CMKC-COP showed the potentiality to repair skin in cosmetic, biomedical and pharmaceutical fields.