Acetic acid as a decontamination method for sink drains in a nosocomial outbreak of metallo-β-lactamase-producing Pseudomonas aeruginosa

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• 作者：A. Stjä ; rne Aspelund^a ; ^{anna.stjarne_aspelund@med.lu.se" class="auth_mail" title="E-mail the corresponding author} ; K. Sjö ; strö ; m^b ; B. Olsson Liljequist^b ; M. Mö ; rgelin^c ; E. Melander^a ; L.I. På ; hlman^d
• 关键词：Metallo-β-lactamase producing Pseudomonas aeruginosa ; Nosocomial outbreak ; Sink drains ; Acetic acid ; Decontamination ; Biofilm
• 刊名：Journal of Hospital Infection
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：94
• 期：1
• 页码：13-20
• 全文大小：1386 K

文摘

Pseudomonas aeruginosa may colonize water systems via biofilm formation. In hospital environments, contaminated sinks have been associated with nosocomial transmission. Here we describe a prolonged outbreak of a metallo-β-lactamase-producing P. aeruginosa (Pae-MBL) associated with sink drains, and propose a previously unreported decontamination method with acetic acid.

d="absSec_2">Aim

d="abspara0015">To describe a nosocomial outbreak of Pae-MBL associated with hospital sink drains and to evaluate acetic acid as a decontamination method.

d="absSec_3">Methods

d="abspara0020">The outbreak was investigated by searching the microbiology database, microbiological sampling and strain typing. Antibacterial and antibiofilm properties of acetic acid were evaluated in vitro. Pae-MBL-positive sinks were treated with 24% acetic acid once weekly and monitored with repeated cultures.

d="absSec_4">Findings

d="abspara0025">Fourteen patients with positive cultures for Pae-MBL were identified from 2008 to 2014. The patients had been admitted to three wards, where screening discovered Pae-MBL in 12 sink drains located in the patient bathrooms. Typing of clinical and sink drain isolates revealed identical or closely related strains. Pae-MBL biofilm was highly sensitive to acetic acid with a minimum biofilm eradication concentration of 0.75% (range: 0.19–1.5). Weekly treatment of colonized sink drains with acetic acid resulted in negative cultures and terminated transmission.

d="absSec_5">Conclusion

d="abspara0030">Acetic acid is highly effective against Pae-MBL biofilms, and may be used as a simple method to decontaminate sink drains and to prevent nosocomial transmission.