The Raver1 was identified from sea perch for the first time. LjRAVER1 transcripts were induced greatly post RGNNV infection in vivo. LjRAVER1 was involved in response to poly(I:C) and RGNNV stimulation in vitro. Overexpression of LjRAVER1 inhibited the replication of RGNNV. The ectopic expression of LjRAVER1 activated RLR signaling pathway.