PP2Acα positively regulates the termination of liver regeneration in mice through the AKT/GSK3β/Cyclin D1 pathway

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• 作者：Shan-Shan Lai¹ ; Dan-Dan Zhao¹ ; Peng Cao² ; ³ ; Ke Lu¹ ; Ou-Yang Luo¹ ; Wei-Bo Chen¹ ; ⁴ ; Jia Liu¹ ; En-Ze Jiang¹ ; Zi-Han Yu¹ ; Gina Lee⁵ ; Jing Li⁵ ; De-Cai Yu⁴ ; Xiao-Jun Xu⁶ ; Min-Sheng Zhu¹ ; Xiang Gao¹ ; ^{gaoxiang@nju.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Chao-Jun Li¹ ; ^{licj@nju.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Bin Xue¹ ; ^{xuebin@nju.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：LR ; liver regeneration ; PH ; partial hepatectomy ; ALT ; alanine aminotransferase ; AST ; aspartate aminotransferase ; GSK3β ; Glycogen synthase kinase-3β ; PP1 ; protein phosphatase1 ; NMR ; nuclear magnetic resonance ; NPCs ; non-parenchymal cells
• 刊名：Journal of Hepatology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：64
• 期：2
• 页码：352-360
• 全文大小：2990 K

文摘

Liver injury triggers a highly organized and ordered liver regeneration (LR) process. Once regeneration is complete, a stop signal ensures that the regenerated liver is an appropriate functional size. The inhibitors and stop signals that regulate LR are unknown, and only limited information is available about these mechanisms.

Methods

A 70% partial hepatectomy (PH) was performed in hepatocyte-specific PP2Acα-deleted (PP2Acα^−/−) and control (PP2Acα^+/+) mice. LR was estimated by liver weight, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and cell proliferation, and the related cellular signals were analyzed.

Results

We found that the catalytic subunit of PP2A was markedly upregulated during the late stage of LR. PP2Acα^−/− mice showed prolonged LR termination, an increased liver size compared to the original mass and lower levels of serum ALT and AST compared with control mice. In these mice, cyclin D1 protein levels, but not mRNA levels, were increased. Mechanistically, AKT activated by the loss of PP2Acα inhibited glycogen synthase kinase 3β (GSK3β) activity, which led to the accumulation of cyclin D1 protein and accelerated hepatocyte proliferation at the termination stage. Treatment with the PI3K inhibitor wortmannin at the termination stage was sufficient to inhibit cyclin D1 accumulation and hepatocyte proliferation.

Conclusions

PP2Acα plays an essential role in the proper termination of LR via the AKT/GSK3β/Cyclin D1 pathway. Our findings enrich the understanding of the molecular mechanism that controls the termination of LR and provides a potential therapeutic target for treating liver injury.