Functional properties of the human ventral mesencephalic neural stem cell line hVM1
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- 作者：Jan Tø ; nnesen ; Emma Gonzalez Seiz ; Milagros Ramos ; Olle Lindvall ; Alberto Martinez-Serrano ; Merab Kokaia
- 关键词：Human neural stem cell ; Dopaminergic neuron ; In vitro ; Electrophysiology ; Functional properties ; Differentiation ; Parkinson's disease
- 刊名：Experimental Neurology
- 出版年：2010
- 出版时间：June 2010
- 年：2010
- 卷：223
- 期：2
- 页码：653-656
- 全文大小：1699 K
文摘
The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiological properties of functional neurons and respond to neurotransmitters in vitro. However, the electrophysiological properties are immature and the cells require longer maturation time than possible under in vitro conditions.