Mass spectrometric characterization of a two-glycosyltransferase tandem reaction for assembly of tetrasaccharide repeating unit of Escherichia coli O77 O-antigen
详细信息 查看全文
- 作者：Dawei Zhoua ; 1 ; Chao Chena ; 1 ; Lingling Xua ; Natalia Utkinab ; Leonid Danilovb ; Vladimir Torgovb ; Vladimir Veselovskyb ; Bin Liua ; Lu Fenga ; fenglu63@nankai.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：E.&thinsp ; coli O77 ; O-antigen repeating unit ; Glycosyltransferases ; WbaD ; WbaC ; Electrospray ionization multistage tandem mass spectrometry
- 刊名：Carbohydrate Research
- 出版年：2016
- 出版时间：7 April 2016
- 年：2016
- 卷：424
- 期：Complete
- 页码：24-29
- 全文大小：434 K
文摘
- •
MS provides valuable information about in vitro characterization of glycosyltransferases.
- •
MS is highly valuable for precise structure elucidation of lipooligosaccharides.
- •
The wbaD gene and wbaC gene from E. coli O77 encoding mannosyltransferases were characterized.
- •
This study leads to complete characterization of O77 O-repeating unit synthetic pathway.