L-type calcium channels play a crucial role in the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

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• 作者：Li Wen ; Yu Wang ; Huan Wang ; Lingmin Kong ; Liang Zhang ; Xin Chen ; Yin Ding
• 关键词：L-type Ca2+ channel ; Mesenchymal stem cells ; Cell proliferation ; Osteogenic differentiation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：3 August, 2012
• 年：2012
• 卷：424
• 期：3
• 页码：439-445
• 全文大小：1295 K

文摘

L-type voltage-dependent Ca²⁺ channels (VDCC_L) play an important role in the maintenance of intracellular calcium homeostasis, and influence multiple cellular processes. They have been confirmed to contribute to the functional activities of osteoblasts. Recently, VDCC_L expression was reported in mesenchymal stem cells (MSCs), but the role of VDCC_L in MSCs is still undetermined. The aim of this study was to determine whether VDCC_L may be regarded as a new regulator in the proliferation and osteogenic differentiation of rat MSC (rMSCs). In this study, we examined functional Ca²⁺ currents (I_Ca) and mRNA expression of VDCC_L in rMSCs, and then suppressed VDCC_L using nifedipine (Nif), a VDCC_L blocker, to investigate its role in rMSCs. The proliferation and osteogenic differentiation of MSCs were analyzed by MTT, flow cytometry, alkaline phosphatase (ALP), Alizarin Red S staining, RT-PCR, and real-time PCR assays. We found that Nif exerts antiproliferative and apoptosis-inducing effects on rMSCs. ALP activity and mineralized nodules were significantly decreased after Nif treatment. Moreover, the mRNA levels of the osteogenic markers, osteocalcin (OCN), bone sialoprotein (BSP), and runt-related transcription factor 2 (Runx2), were also down-regulated. In addition, we transfected ¦Á1C-siRNA into the cells to further confirm the role of VDCC_L in rMSCs, and a similar effect on osteogenesis was found. These results suggest that VDCC_L plays a crucial role in the proliferation and osteogenic differentiation of rMSCs.