Modification of agarose with carboxylation and grafting dopamine for promotion of its cell-adhesiveness

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• 作者：Su Yixue ; Chu Bin ; Gao Yuan ; Wu Chaoxi ; Zhang Lingmin ; Chen Peng ; Wang Xiaoying ; Tang Shunqing ; ^{tshunqt@jnu.edu.cn}
• 关键词：Agarose ; Dopamine ; Graft ; Cell-adhesiveness
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：92
• 期：2
• 页码：2245-2251
• 全文大小：1345 K

文摘

In order to improve bioactivity of agarose, we modified agarose by carboxylation and grafting dopamine. Under alkaline condition, carboxylated agarose was prepared using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) oxidation system by oxidizing C₆ hydroxyl on d-galactose ring into carboxyl group, and the maximum value of the degree of carboxylation reached 30 % . With the increase of the amount of oxidant, the molecular weight of the carboxylated agarose decreased to 4 kDa by gel permeation chromatography (GPC) measure. Carboxylated agarose reacted with dopamine through EDC condensation reaction to obtain agarose grafting dopamine (Ag-g-DA), and the grafting rate of dopamine was determined to be 9.3 % by UV spectroscopy at 280 nm. The structures of these modified agaroses were determined by FT-IR and ¹³C NMR. Both carboxylated agarose and Ag-g-DA showed no cytotoxicity and promoted cell-adhesiveness.